Kisspeptin-10 is a decapeptide fragment of the KISS1 gene product and the shortest naturally occurring sequence that fully activates the kisspeptin receptor (KISS1R, also called GPR54). Studied as a master regulator of the reproductive neuroendocrine axis, it is a central tool for investigating GnRH neuron activation and gonadotropin signaling in experimental systems. Puritide Research supplies Kisspeptin-10 as a high-purity, research-use-only material, synthesized for in vitro and animal-model work and backed by third-party-verified certificates of analysis.
Available Sizes
- ›Kisspeptin-10 10 mg Vial
Product Specifications
- Application
- Reproductive neuroendocrine and KISS1R/GPR54 receptor signaling research
- Appearance
- Solid, white lyophilized powder in a sealed glass vial
- Chemical Formula
- C63H83N17O14
- PubChem SID
- 25240297
- CAS Number
- 374675-21-5
- Molecular Weight
- ~1302.45 g/mol
- Synonyms
- Kisspeptin-10, Metastin (45-54), KISS1 (112-121), Kp-10
- Storage
- Store at ≤25°C, sealed, away from heat, light, and moisture. For extended storage, keep lyophilized and refrigerated or frozen.
What Is Kisspeptin-10?
Kisspeptin-10 is a synthetic decapeptide (sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2) corresponding to residues 112-121 of the KISS1 gene product. Among the several kisspeptin fragments derived from the parent protein, the decapeptide is reported to be the most potent at activating the receptor KISS1R (GPR54).
Kisspeptin signaling has been identified as a key upstream regulator of gonadotropin-releasing hormone (GnRH) neurons, positioning it as a master controller of puberty and reproductive function in mammalian models. Kisspeptin-10 is therefore widely used to probe the neuroendocrine circuitry governing fertility.
Present understanding derives from in vitro assays, animal models, and human research. Findings should be interpreted within their experimental context and not treated as established clinical outcomes. Puritide Research supplies Kisspeptin-10 strictly for laboratory use; it is not approved for human or veterinary application.
Kisspeptin-10 Mechanism of Action (Research Only)
Kisspeptin-10 is studied for its agonism of the KISS1R (GPR54) receptor and the resulting activation of GnRH neurons that sit at the top of the reproductive axis. The observations below reflect biochemical assays, cell-culture studies, and animal models, and should be read strictly in a research context.
KISS1R (GPR54) Receptor Agonism
Kisspeptin-10 binds and activates KISS1R, a G-protein-coupled receptor. Activation is associated with Gq/11 signaling, phospholipase C activity, and intracellular calcium mobilization in experimental systems, events linked to the depolarization and firing of receptor-expressing neurons.
GnRH Neuron Activation and Gonadotropin Signaling
Kisspeptin-expressing neurons project onto GnRH neurons, and kisspeptin signaling is studied as essential for GnRH neuron activation and the preovulatory luteinizing hormone surge in animal models. Loss-of-function mutations in GPR54 are associated with hypogonadotropic hypogonadism, underscoring the pathway's central role.
Researchers use Kisspeptin-10 to characterize receptor pharmacology, GnRH-neuron responses, and downstream gonadotropin (LH/FSH) signaling under defined conditions, and to compare it with longer kisspeptin fragments such as Kisspeptin-54, rather than to establish physiological effects.
Kisspeptin-10 Research Applications
Kisspeptin-10 is investigated across neuroendocrine and preclinical studies as a probe of the reproductive axis. The observations below derive from biochemical studies, cell-based assays, and animal models, and should be interpreted within their respective settings.
Reproductive Neuroendocrine Models
A primary application is investigating KISS1R-mediated activation of GnRH neurons and the resulting gonadotropin (LH and FSH) signaling in animal and cell models. Kisspeptin-10 is used to study the timing of the LH surge, ovulation-related circuitry, and the onset of pubertal signaling.
It is also applied in comparative research alongside Kisspeptin-54 and GnRH, examining differences in potency, signaling duration, and receptor engagement without establishing clinical outcomes.
Receptor Pharmacology and Metabolic Cross-Talk
Kisspeptin-10 is used to characterize KISS1R receptor pharmacology in transfected cell systems, including binding, signaling, and desensitization. Researchers also study kisspeptin signaling at the interface of reproductive and metabolic regulation. These studies aim to clarify receptor-mediated communication rather than to establish therapeutic effects.
Laboratory Safety & Handling (Research Use Only)
Kisspeptin-10 is a synthetic, lyophilized research peptide for laboratory investigation only. It should be handled by qualified personnel in controlled environments, in accordance with institutional policies, laboratory safety procedures, and applicable regulations. As an investigational material, laboratories should implement practices that prioritize material integrity, traceability, and reproducibility.
Handling & Storage Guidelines
Follow institutional SOPs, chemical hygiene plans, and approved protocols; wear appropriate PPE (lab coat, gloves, eye protection); and use engineering controls where required. Record lot numbers, storage conditions, and handling activities, and retain Certificates of Analysis and batch documentation to support traceability and audit readiness.
Store material under the conditions specified in product documentation, protected from excessive heat, moisture, and direct light. Maintain consistent conditions throughout the study period and monitor storage records as part of quality assurance. Consistent handling and documentation support reliable, reproducible outcomes.
Certificate of Analysis & Quality Assurance
Each Kisspeptin-10 batch ships with a third-party-verified Certificate of Analysis, providing transparent, research-grade quality control. The CoA includes a purity assessment (HPLC / reverse-phase chromatography), mass-spectrometry identity confirmation, and lot number, testing date, and method documentation.
Researchers may request sample CoAs (PDF) ahead of purchase to confirm testing parameters, analytical methods, and batch consistency, and are encouraged to perform independent verification.
Research Use Only
